Reconstitution of glycophosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco

Authors :: Bao-Qing Dun
Xu-Jing Wang
Wei Lu
Zhong-Lin Zhao
Song-Na Hou
Bao-Ming Zhang
Gui-Ying Li
Evans, Thomas C., Jr.
Ming-Qun Xu
Min Lin
Source :: Applied and Environmental Microbiology. Dec, 2007, Vol. 73 Issue 23-24, p7997, 4 p.
Publication Year :: 2007
Abstract: A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) is mapped in order to identify potential split sites by using a transposon-based linker-scanning method. Intein-mediated protein complementation is used for reconstituting glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.

Subjects :: Genetically modified crops -- Research
Escherichia coli -- Research
Escherichia coli -- Genetic aspects
Biological sciences

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