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MOSFET-based pulse power supply for bacterial transformation
- Source :
- IEEE Transactions on Industry Applications. Jan-Feb, 2008, Vol. 44 Issue 1, p25, 7 p.
- Publication Year :
- 2008
-
Abstract
- In this paper, a MOSFET-based pulsed power supply capable of supplying square pulses of up to 3000 V and widths from nanoseconds to milliseconds is presented and used for an investigation into electroporation-mediated delivery of a plasmid DNA molecule into the pathogenic bacterium Escherichia coli O157:H7. It was concluded that increasing the electric field strength and pulse amplitude resulted in an increase in the number of transformants. However, increasing the number of pulses had the effect of reducing the number of transformants. In all the experiments, the number of calls that were inactivated by the exposure to the pulsed electric field were also measured. Index Terms--Bacterial transformation, DNA transfusion, electroporation, Escherichia coli.
- Subjects :
- Business
Computers
Electronics
Electronics and electrical industries
Subjects
Details
- Language :
- English
- ISSN :
- 00939994
- Volume :
- 44
- Issue :
- 1
- Database :
- Gale General OneFile
- Journal :
- IEEE Transactions on Industry Applications
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.174973227