G-tensors of the flavin adenine dinucleotide radicals in glucose oxidase: a comparative multifrequency electron paramagnetic resonance and electron-nuclear double resonance study

A study is conducted to characterize the flavin adenine dinucleotide (FAD) cofactor of Aspergillus niger glucose oxidase (GO) in its anionic and neutral radical form. The g-tensor reflects more global changes in the electronic structure of flavin, hence could be applied to identify chemically different flavin radicals.