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In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa
- Source :
- Journal of the American Chemical Society. April 9, 2008, Vol. 130 Issue 14, 4954-14
- Publication Year :
- 2008
-
Abstract
- D-Forosamine biosynthesis is studied by using the five genes from the spinosyn gene cluster involved in the conversion ofTDP-4-keto-6-deoxy-D-glucose to TDP-D-forosamine. The steady-state kinetic parameters for the SpnQ-catalyzed reaction and the substrate specificities of SpnQ and SpnR are explored.
Details
- Language :
- English
- ISSN :
- 00027863
- Volume :
- 130
- Issue :
- 14
- Database :
- Gale General OneFile
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.178171449