Real-time PCR assays for quantification and differentiation of Vibrio vulnificus strains in oysters and water

Real-time PCR assays developed for detecting and differentiating two 16S rRNA variants of Vibrio vulnificus in oysters and water is reported. These assays were found to be specific and sensitive in differentiating the strains of V. vulnificus in oysters without requiring its isolation and may be useful in quick detection of pathogens in shellfish and water.