Yersinia spp. HMWP2, a cytosolic protein with a cryptic internal signal sequence which can promote alkaline phosphatase export

The TnphoA mutagenesis, to create a number of irp2-phoA gene fusions on a multicopy number plasmid, and then by determining the conditions under which HMWP2 does not form disulfide-bonded aggregates when the cells were disrupted are the processes which proved that HMWP2 is a predominantly soluble cytoplasmic protein with a cryptic internal export signal in Yersinia enterocolitica. This high alkaline phosphatase activity is associated with some of the HMWP2-PhoA hybrids.