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Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N- or C-terminal end
- Source :
- Journal of Bacteriology. Sept, 1995, Vol. 177 Issue 17-18, p5238, 9 p.
- Publication Year :
- 1995
-
Abstract
- Pullulanase secretion from Escherichia coli containing secretion genes from Klebsiella oxytoca was not inhibited by 23 separate linker insertions of 2-4 codons each, but was inhibited by fusion of reporter proteins to either the N- or C-terminus. Shortening one reporter protein, however, did permit secretion. Eleven of the insertions abolished pullulanase activity. The reporter hybrids did not alter activity, suggesting that pullulanase conformation was not significantly changed in these hybrids.
Details
- ISSN :
- 00219193
- Volume :
- 177
- Issue :
- 17-18
- Database :
- Gale General OneFile
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.17928521