Characterization of the Fe site in iron-sulfur cluster-free hydrogenase (Hmd) and of a model compound via nuclear resonance vibrational spectroscopy (NRVS)

Authors :: Yisong Guo
Hongxin Wang
Yuming Xiao
Vogt, Sonja
Thauer, Rudolf K.
Seigo Shima
Volkers, Philip I.
Rauchfuss, Thomas B.
Pelmenschikov, Vladimir
Case, David A.
Alp, Ercan E.
Wolfgang Sturhahn
Yoda, Yoshitaka
Cramer, Stephen P.
Source :: Inorganic Chemistry. May 19, 2008, Vol. 47 Issue 10, 3969-3977
Publication Year :: 2008
Abstract: A [super 57]Fe nuclear resonance vibrational spectroscopy (NRVS) was used to study the iron site in the iron-sulfur cluster-free hydrogenase Hmd from the methanogenic archaeon Methanothermobacter marburgensis. The data obtained for NRVS provided insights into the dynamics of the Fe site, revealing Fe-CO stretch and Fe-CO bend modes at 494, 562, 590, and 648 [cm.sup.-1], which are consistent with the proposed cis-Fe[(CO).sub.2] ligation.

Subjects :: Hydrogenation -- Analysis
Methanobacteriaceae -- Research
Spectrum analysis -- Usage
Chemistry

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