Human uridine monophosphate synthase: baculovirus expression, immunoaffinity column purification and characterization of the acetylated amino terminus

The N-terminal sequence of pure human uridine monophosphate synthase is modified in vitro and that changes involve removal of N-terminal methionine residue from the gene-coded sequence and subsequent acetylation of the formerly penultimate alanine residue to yield acetyl-AVAR as the acetylated N-terminal tetrapeptide. Purified protein expressed in cabbage looper (Tricoplusiani) larvae expressing human cDNA also begins with the N-terminal acetyl-AVAR.