Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase

A method using notable protein labeling technology is described that can be used to covalently immobilize proteins of interest site-specifically in a single, efficient enzymatic ligation step. The phosphospantetheinyl transferase Sfp-catalyzed surface ligation is mild, quantitative and rapid, which has occurred in a single step without prior chemical modification of the target protein.