Purinergic receptor stimulation increases membrane trafficking in brown adipocytes

The exposure of brown adipocytes to extracellular ATP leads to exocytosis of intracellular membranes and increases the purinergic receptor mediated membrane trafficking. There is also a purinergic stimulated increase in the cell surface due to adipocyte vesicle-mediated secretion. The ATP exposure also increases the total cell membrane capacitance and the cytosolic calcium (Ca) levels. Adrenergic activation mobilizes calcium without influencing the amount of membrane trafficking. This indicates that the increase in Ca content is not solely responsible for the ATP-induced cellular responses.