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Magnetic beads based immunoaffinity capillary electrophoresis of total serum IgE with laser-induced fluorescence detection
- Source :
- Analytical Chemistry. Dec 15, 2008, Vol. 80 Issue 24, p9583, 6 p.
- Publication Year :
- 2008
-
Abstract
- A magnetic beads based immunoaffinity capillary electrophoresis method for total Immunoglobulin E quantification in serum has been developed. The method combines speed, automation ability, and minimal sample consumption. Only 1 [micro]L of serum is required while the whole immunoaffinity capillary electrophoresis method is performed in less than 50 min. The concomitant use of online immunocapture, transient isotachophoresis, and laser-induced fluorescence detection provides a sensitivity in the low picomolar range and a highly linear fluorescence response over 4 orders of magnitude (IgE concentration ranging from 2.4 to 2400 ng/mL). After validation with a reference material, the method has been successfully applied to the quantification of total IgEs in patient sera. The results compared well with classical ImmunoCap data.
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 80
- Issue :
- 24
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.191514032