Thrombin-mediated increases in cytosolic [[Ca.sup.2+]] involve different mechanisms in human pulmonary artery smooth muscle and endothelial cells

Thrombin is a procoagulant inflammatory agonist that can disrupt the endothelium-lumen barrier in the lung by causing contraction of endothelial cells and promote pulmonary cell proliferation. Both contraction and proliferation require increases in cytosolic [Ca.sup.2+] concentration ([[[Ca.sup.2+]].sub.cyt]). In this study, we compared the effect of thrombin on [Ca.sup.2+] signaling in human pulmonary artery smooth muscle (PASMC) and endothelial (PAEC) cells. Thrombin increased the [[[Ca.sup.2+]].sub.cyt] in both cell types; however, the transient response was significantly higher and recovered quicker in the PASMC, suggesting different mechanisms may contribute to thrombin-mediated increases in [[[Ca.sup.2+]].sub.cyt] in these cell types. Depletion of intracellular stores with cyclopiazonic acid (CPA) in the absence of extracellular [Ca.sup.2+] induced calcium transients representative of those observed in response to thrombin in both cell types. Interestingly, CPA pretreatment significantly attenuated thrombin-induced [Ca.sup.2+] release in PASMC; this attenuation was not apparent in PAEC, indicating that a PAEC-specific mechanism was targeted by thrombin. Treatment with a combination of CPA, caffeine, and ryanodine also failed to abolish the thrombin-induced [Ca.sup.2+] transient in PAEC. Notably, thrombin-induced receptor-mediated calcium influx was still observed in PASMC after CPA pretreatment in the presence of extracellular [Ca.sup.2+]. [Ca.sup.2+] oscillations were triggered by thrombin in PASMC resulting from a balance of extracellular [Ca.sup.2+] influx and [Ca.sup.2+] reuptake by the sarcoplasmic reticulum. The data show that thrombin induces increases in intracellular calcium in PASMC and PAEC with a distinct CPA-, caffeine-, and ryanodine-insensitive release existing only in PAEC. Furthermore, a dynamic balance between [Ca.sup.2+] influx, intracellular [Ca.sup.2+] release, and reuptake underlie the [Ca.sup.2+] transients evoked by thrombin in some PASMC. Understanding of such mechanisms will provide an important insight into thrombin-mediated vascular injury during hypertension. sarcoplasmic and endoplasmic reticulum; [Ca.sup.2+] store