Analysis of mechanisms regulating expression of the ver-1 gene involved in aflatoxin biosynthesis

A systematic analysis of aflatoxin biosynthesis was conducted in Aspergillus parasiticus to determine the molecular mechanisms that regulates the accumulation of proteins encoded by aflatoxin biosynthetic genes. Analysis of aflatoxin biosynthesis in Aspegillus parasiticus indicated the similar appearance of GUS transcript and GUS proteins with the accumulation of ver-1A transcript and Ver-1 protein. Furthermore, gene expression levels in Aspergillus parasiticus are dependent on chromosomal location and the mechanisms for gene inactivation exists in the fungi strain.