NMR studies of the paramagnetic complex Fe(II)-bleomycin

NMR studies of the paramagnetic Fe(II)BLM identified the ligands to the metal center in the Fe(II) adduct of bleomycin as derived from the beta-aminoalanine, the pyrimidinylpropionamide and the beta-hydroxyhistidine segments. Two-dimensional NMR techniques also allowed the identification of many of the spin networks present in the NMR spectrum of the paramagnetic Fe(II)BLM. Moreover, they clarify the assignments for the signals between -0.8 and -20 ppm. Examination of the NMR data reveals that alpha-methylvalerate and/or mannose segments do not participate in Fe(II) complexation.