Discovery of stable and variable differences in the Mycobacterium avium subsp. paratuberculosis type I, II, and III genomes by pan-genome microarray analysis

Authors :: Castellanos, Elena
Aranaz, Alicia
Gould, Katherine A.
Linedale, Richard
Stevenson, Karen
Alvarez, Julio
Dominguez, Lucas
de Juan, Lucia
Hinds, Jason
Bull, Tim J.
Source :: Applied and Environmental Microbiology. Feb, 2009, Vol. 75 Issue 3-4, 676-686
Publication Year :: 2009
Abstract: The design, validation, and application of the MAPAC pan-genome microarray consisting of optimized oligonucleotide reporters to generate a specific signal for each of the shared and unique ORFs present in the Mycobacterium avium subsp. hominissuis 104 and M. avium subsp. paratuberculosis K-10 genomes are described. Quantitative PCR has shown that variation in vGI signals is associated with colony growth rate and morphology.

Subjects :: Crohn's disease -- Causes of
DNA microarrays -- Usage
Gene mutations -- Analysis
Mycobacterium avium -- Genetic aspects
Polymerase chain reaction -- Usage
Biological sciences

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