Characterization of serine proteinase expression in Agaricus bisporus and Coprinopsis cinerea by using green fluorescent protein and the A. bisporus SPR1 promoter

An serine bisporus (SPR) promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, is constructed to study the temporal and developmental expression of the Agaricus bisporus SPR 1 (SPR1) in A. bisporus and Coprinopsis cinerea. The results obtained confirmed that the A. bisporus promoter (SPR1) is able to regulate mycelial serine proteinase production in response to specific nitrogen sources and have tissue specific expression in detached sporophores.