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Overexpression and characterization of a prolyl endopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus
- Source :
- Journal of Bacteriology. June, 1997, Vol. 179 Issue 11-12, p3613, 6 p.
- Publication Year :
- 1997
-
Abstract
- The maltose-regulated mlr-2 gene from the hyperthermophilic archaeon Pyrococcus furiosus was cloned and overexpressed in Escherichia coli. The mlr-2 gene has homology to bacterial and eukaryal prolyl endopeptidase (PEPase). The mlr-2 gene was renamed prpA and its product was purified and characterized. Findings indicated that PrpA has PEPase enzymatic activity. The maltose-dependent regulation of PEPase in P. furiosus was studied and revealed that enzyme activity varies in the same way as prpA mRNA.
Details
- ISSN :
- 00219193
- Volume :
- 179
- Issue :
- 11-12
- Database :
- Gale General OneFile
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.20009129