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Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification
- Source :
- Applied and Environmental Microbiology. August, 1997, Vol. 63 Issue 8, p3268, 6 p.
- Publication Year :
- 1997
-
Abstract
- In-situ hybridization has found increasing use in nucleic acid probes in studying the specific sequences of whole-fixed bacterial cells. The use of fluorescein-tyramide as a substrate for oligonucleotides labeled with horseradish peroxidase can improve the in situ hybridization of bacterial cells. Flow cytometry measurements show that the hybridized cells have a 10- to 20-fold signal amplifications.
Details
- ISSN :
- 00992240
- Volume :
- 63
- Issue :
- 8
- Database :
- Gale General OneFile
- Journal :
- Applied and Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.20034294