Interaction of fibrin(ogen) with the endothelial cell receptor ve-cadherin: localization of the fibrin-binding site within the third extracellular ve-cadherin domain

Several recombinant fragments containing individual extracellular domains of VE-cadherin and several fragments corresponding to various fibrin(ogen) regions are prepared to test the interactions involved in fibrin(ogen) region/domains and localize the complementary fibrin-binding site in VE-cadherin. The experimental results confirmed that the fibrin-binding properties of the VE-cadherin are not influenced by the calcius ions that are required to maintain proper conformation and adhesive properties of the VE-cadherin.