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Inhibition of transcription in Staphylococcus aureus by a primary sigma factor-binding polypeptide from phage G1
- Source :
- Journal of Bacteriology. June, 2009, Vol. 191 Issue 11-12, p3763, 9 p.
- Publication Year :
- 2009
-
Abstract
- The primary sigma factor of Staphylococcus aureus, [[sigma].sup.SA], regulates the transcription of many genes, including several essential genes, in this bacterium via specific recognition of exponential growth phase promoters. In this study, we report the existence of a novel staphylococcal phage G1-derived growth inhibitory polypeptide, referred to as G1ORF67, that interacts with [[sigma].sup.SA] both in vivo and in vitro and regulates its activity. Delineation of the minimal domain of [[sigma].sup.SA] that is required for its interaction with G1ORF67 as amino acids 294 to 360 near the carboxy terminus suggests that the G1 phage-encoded anti-[sigma] factor may occlude the -35 element recognition domain of [[sigma].sup.SA]. AS would be predicted by this hypothesis, the G1ORF67 polypeptide abolished both RNA polymerase core-dependent binding of [[sigma].sup.SA] to DNA and [[sigma].sup.SA]-dependent transcription in vitro. While G1ORF67 profoundly inhibits transcription when expressed in S. aureus cells in mode of action studies, our finding that G1ORF67 was unable to inhibit transcription when expressed in Escherichia coli concurs with its inability to inhibit transcription by the E. coli holoenzyme in vitro. These features demonstrate the selectivity of G1ORF67 for S. aureus RNA polymerase. We predict that G1ORF67 is one of the central polypeptides in the phage G1 strategy to appropriate host RNA polymerase and redirect it to phage reproduction.
- Subjects :
- Staphylococcus aureus -- Genetic aspects
Staphylococcus aureus -- Research
Genetic transcription -- Research
Polypeptides -- Physiological aspects
Polypeptides -- Genetic aspects
Polypeptides -- Research
RNA polymerases -- Research
Bacteriophages -- Genetic aspects
Bacteriophages -- Research
Bacterial genetics -- Reports
Biological sciences
Subjects
Details
- Language :
- English
- ISSN :
- 00219193
- Volume :
- 191
- Issue :
- 11-12
- Database :
- Gale General OneFile
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.202487142