Relationship of two apolipoprotein B polymorphisms with serum lipoprotein and lipid levels in African Blacks

Apolipoprotein B (apoB, protein; APOB, gene) is one of a dozen proteins associated with plasma lipoprotein particles and thus plays a crucial role in lipid metabolism. In recent years many [...]
Two DNA polymorphisms, insertion/deletion (ins/del) and XbaI, of the apolipoprotein B (APOB) gene and their relationships with serum lipoprotein and lipid levels were examined in two Nigerian populations from Benin (n = 828) and Sokoto (n = 394). Allele frequencies for both polymorphisms were comparable between the two populations, and genotype frequencies conformed to Hardy-Weinberg equilibrium expectations. Because lipid profiles are affected by many known sex differences, males and females were analyzed separately. Analysis of variance was performed to test equality of means of quantitative traits across genotypes; the dependent variables were first adjusted by stepwise linear regression for environmental covariates in both populations. Plasma apoB and HDL2 cholesterol levels varied significantly between ins/del genotypes in Benin males (p = 0.05) and Sokoto males (p = 0.02), respectively. No significant variation between XbaI genotypes was observed for any lipid trait in either population. Combining the ins/del and XbaI polymorphisms to form haplotypes revealed significant linkage disequilibrium in both the Benin (p < 0.0001) and Sokoto (p < 0.001) populations. Analysis of the two-site haplotype showed a significant effect on serum lipoprotein (a) levels in Benin females (p < 0.04) and on HDL2 cholesterol among Sokoto males (p = 0.01). The haplotype explained 4% of the adjusted phenotypic variation in lipoprotein (a) levels in Benin females and 8.5% of the adjusted phenotypic variation in HDL2 cholesterol levels in Sokoto males. These data indicate that the ins/del and XbaI polymorphisms in the APOB gene affect interindividual variation in serum lipoprotein and lipid levels in African populations. KEY WORDS: APOLIPOPROTEIN B, AFRICAN BLACKS, CHOLESTEROL, ALLELLE FREQUENCY, INSERTION/DELETION, XbaI, LINKAGE DISEQUILIBRIUM