Quantification of human polyomaviruses JC virus and BK by TaqMan quantitative PCR and comparison to other water quality indicators in water and fecal samples

A TaqMan quantitative PCR (QPCR) assay is developed and optimized based on the conserved T antigen in order to quantify and detect two human polyomaviruses (HPyVs), JC virus and BK virus. The use of this quantitative assay in water quality research might help regulatory agencies in identifying sources of water pollution for improved remediation of contaminated waters and thus protecting humans from exposure to pathogens.