A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis

Research was conducted to test whether PGC-1 nuclear cell receptor plays an important role in linking nuclear receptors to the transcriptional program of adaptive thermogenesis. Amino acids were cloned in-frame into the DNA-binding domain plasmid pAS2 while an expression plasmid for full-length PGC-1 was established by ligating an entire cDNA as a Smal-Xhol fragment. Results indicated that nuclear cell receptors control a range of physiological processes in metazoan organisms.