Back to Search
Start Over
Transposition into Replicating DNA Occurs through Interaction with the Processivity Factor
- Source :
- Cell. August 21, 2009, Vol. 138 Issue 4, p685, 11 p.
- Publication Year :
- 2009
-
Abstract
- To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.cell.2009.06.011 Byline: Adam R. Parks (1), Zaoping Li (1), Qiaojuan Shi (1), Roisin M. Owens (2), Moonsoo M. Jin (2), Joseph E. Peters (1) Keywords: DNA; PROTEINS; MICROBIO Abstract: The bacterial transposon Tn7 directs transposition into actively replicating DNA by a mechanism involving the transposon-encoded protein TnsE. Here we show that TnsE physically and functionally interacts with the processivity factor of the DNA replication machinery in vivo and in vitro. Our work establishes an in vitro TnsABC+E transposition reaction reconstituted from purified proteins and target DNA structures. Using the in vitro reaction we confirm that the processivity factor specifically reorders TnsE-mediated transposition events on target DNAs in a way that matches the bias with active DNA replication in vivo. The TnsE interaction with an essential and conserved component of the replication machinery, and a DNA structure reveals a mechanism by which Tn7, and probably other elements, selects target sites associated with DNA replication. Author Affiliation: (1) Department of Microbiology, Cornell University, Ithaca, NY 14853, USA (2) Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA Article History: Received 28 April 2008; Revised 23 March 2009; Accepted 1 June 2009 Article Note: (miscellaneous) Published: August 20, 2009
Details
- Language :
- English
- ISSN :
- 00928674
- Volume :
- 138
- Issue :
- 4
- Database :
- Gale General OneFile
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.206397533