HMW1 is required for cytahedsin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae

The contribution of HMW1 in the cytadherence of the M6 mutant was investigated via genetic manipulation. The introduction of a recombinant transposon led to the transformation of the mutant. The transformants were found to synthesize the truncated P30 from the resident allele, while the full-length P30 was synthesized from the recombinant alleles. The results further revealed that the transformants were constantly hemadsorption negative, indicating that HMW1 is essential for cytadherence.