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Role of the omega-loop in the activity, substrate specificity, and structure of class A beta-lactamase
- Source :
- Biochemistry. March 10, 1998, Vol. 37 Issue 10, p3286, 11 p.
- Publication Year :
- 1998
-
Abstract
- The effect of the omega-loop deletion in the function and structure of the enzyme beta-lactamases was investigated. A beta-lactamase mutant protein was produced using the four-primer overlap-extension method. A double-stranded plasmid DNA was then used to resequence the beta-lactamase gene. It was found that the absence of the omega-loop eliminated Glu166, a primary catalytic residue, thus resulting in dysfunctional deacylation. It also eliminated the enzyme's ability to hydrolyze beta-lactam compounds.
Details
- ISSN :
- 00062960
- Volume :
- 37
- Issue :
- 10
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.20998871