Regulation of adenylyl cyclase in polarized renal epithelial cells by G protein-coupled receptors

Research shows that biochemical adenylyl cyclase activity occurs in the basolateral and apical regions of bovine renal epithelial LLC-PK1 cells. Experiments designed to demonstrate the regulatory properties of adenylyl cyclase via Guanine protein-coupled nucleotide binding receptors are described. Immunolocalization and immunofluorescence techniques are used.