Oxidase activity of the acyl-CoA dehydrogenases

Research was conducted to examine the oxidase activity of the acyl-CoA dehydrogenases. A second chromophoric substrate of the medium chain enzyme was used and another representative of the acyl-CoA dehydrogenase family was tested. Results indicate that oxygen interacts not only with the free enzyme but also with several reduced enzyme species. These results underscore the complexity of an apparently simple reaction as the reoxidation of the acyl-CoA dehydrogenase by molecular oxygen.