A novel protein complex that interacts with the vitamin D3 receptor in a ligand-dependent manner and enhances VDR transactivation in a cell-free system

Existence of a complex of novel functional nuclear receptor coactivators not known before has been shown. The vitamin D3 receptor ligand-binding domain (VDR LBD) has been used as an affinity matrix to identify parts of a nuclear extract active in transcription. As a response to ligand, the components interact with VDR. At least 10 VDR interacting proteins (DRIPs) from 65 to 250 kD associate in a complex with the receptor in a way that is strictly dependent on 1,25-dihydroxyvitamin D3.