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Topography of the interaction of HPr(Ser) kinase with HPr
- Source :
- Biochemistry. August 25, 1998, Vol. 37 Issue 34, p11762, 9 p.
- Publication Year :
- 1998
-
Abstract
- Research was conducted to identify the specificity requirements for phosphorylation of Mycoplasma capricolum HPr. A mutagenesis method was used in which residues 43-57 in a chimera were replaced by the Escherichia coli sequence. Results indicate that the kinase-HPr interaction takes place mostly through a hydrophobic region. Comparisons conducted between the chimeric construct and M. capricolum provide a basis for the interpretation of activity assays results.
Details
- ISSN :
- 00062960
- Volume :
- 37
- Issue :
- 34
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.21142364