Ablation of cerebellar Golgi cells disrupts synaptic integration involving GABA inhibition and NMDA receptor activation in motor coordination

Golgi cells expressing the human interleukin-2 receptor alpha subunit in transgenic mice underwent a novel cell ablation technology called immunotoxin-mediated cell targeting (IMCT) since the role played by Golgi cells in cerebellar function and motor coordination was unclear. The conditional disruption of the Golgi cells was through the administration of anti-TAC(Fv)-PE38 recombinant protein intrathecally. It was revealed that Golgi cell elimination resulted to severe motor disorders and weakening of the functional neurotransmitters needed in compound motor coordination.