Herpes simplex virus-1 helicase-primase: roles of each subunit in DNA binding and phosphodiester bond formation

A series of kinetic and photo-cross-linking studies were conducted to gain insight into the roles of each subunit in DNA binding and primer synthesis for the helicase-primase complex from herpes simplex virus-1 contains three subunits, UL5, UL52, and UL8. Photo-cross-linking of a RNA primer-template, the product of primer synthesis, containing 5-iodouracil in the template were shown to generate the same apparent cross-linked species.