Molecular epidemiological studies of Giardiasis in cattle

We conducted the molecular epidemiological study of cattle in Lahore from August 2007 to July 2008. Faecal samples were collected from four different areas, i.e. Military dairy farm, Government dairy farm, Gawala dairy colonies and House hold dairy animals. The faecal samples were purified and the number of cysts purified and quantified. DNA was extracted from the purified cyst and was amplified through PCR. The study revealed the prevalence 31.11% ( 224/720), amongst these the high prevalence was recorded in Govt. dairy farm (46.11%), followed by Gawala dairy colonies (36.11%), Military dairy farm (25.55%) and lowest was recorded in household dairy animals (16.66%). The age-specific herd prevalence was (41.66%) and (27.59%) young and older cattle, accordingly. The high prevalence was observed in female (31.71%) as compare to the male (26.25%). The season wise prevalence was recorded higher in autumn (36.66%) followed by summer (34.58%), spring (30%) and lowest was recorded in winter (25%).The statistical analysis revealed the higher significant in all areas and also in age, sex and season wise (p Keywords: Giardia | Cattle: Prevalence | PCR
Introduction Giardia lamblia, also called Giardia duodenalis or Giardia intestinalis, is a protozoan parasite of the small intestine that causes extensive morbidity worldwide. Giardia was organism seen under the microscope [...]