Tertiary structure and characterization of a glycoside hydrolase family 44 endoglucanase from Clostridium acetobutylicum

Authors :: Warner, Christopher D.
Hoy, Julie A.
Shilling, Taran C.
Linnen, Michael J.
Ginder, Nathaniel D.
Ford, Clark F.
Honzatko, Richard B.
Reilly, Peter J.
Source :: Applied and Environmental Microbiology. Jan, 2010, Vol. 76 Issue 1-2, 338-346
Publication Year :: 2010
Abstract: A gene encoding a glycoside hydrolase family 44 (GH44) proteins from Clostridium acetobutylicum ATCC 824 is prepared and transformed into Escherichia coli. Crystallization and X-ray diffraction have shown a triose phosphate isomerase (TIM) barrel-like structure with additional Greek key and [beta]-sandwich folds, which is similar to other GH44 crystal structures.

Subjects :: Clostridium -- Physiological aspects
Clostridium -- Genetic aspects
Glycosides -- Chemical properties
Isomerases -- Structure
Isomerases -- Chemical properties
Phosphates -- Chemical properties
X-rays -- Diffraction
X-rays -- Usage
Biological sciences

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