Phytochrome as molecular machine: revealing chromophore action during the Pfr to Pr photoconversion by magic-angle spinning NMR spectroscopy

Authors :: Rohmer, Thierry
Lang, Christina
Bongards, Christian
Gupta, Karthick Babu Sai Sankar
Neugebauer, Johannes
Hughes, Jon
Gartner, Wolfgang
Matysik, Jorg
Source :: Journal of the American Chemical Society. March 31, 2010, Vol. 132 Issue 12, 4431-4437
Publication Year :: 2010
Abstract: Magic-angle spinning (MAS) NMR spectroscopy was used to understand the photochemically induced Pfr to Pr back-reaction at low temperature. Uniformly labeled [super 13]C- and [super 15]N-open-chain tetrapyrrole chromophores was used to structurally characterize all four states of Pfr, Lumi-F, Meta-F, and Pr pf cyanobacterial phytochrome Cph1.

Subjects :: Nuclear magnetic resonance spectroscopy -- Usage
Praseodymium -- Chemical properties
Praseodymium -- Structure
Praseodymium -- Spectra
Phytochrome -- Analysis
Chemistry

Tools