Identification and analysis of the biosynthetic gene cluster encoding the thiopeptide antibiotic cyclothiazomycin in Streptomyces hygroscopicus 10-22

Cloning, sequencing, and heterologous expression of the cyclothiazomycin biosynthetic gene cluster from Streptomyces hygroscopicus 10-22 is reported. The rigorous bioinformatics analysis based on heterologous expression of cyclothiazomycin led to an ideal biosynthetic model to better understand the biosynthesis of thiopeptides.