Back to Search Start Over

High-resolution differential ion mobility separations using helium-rich gases

Authors :
Shvartsburg, Alexandre A.
Danielson, William F.
Smith, Richard D.
Source :
Analytical Chemistry. March 15, 2010, Vol. 82 Issue 6, p2456, 7 p.
Publication Year :
2010

Abstract

Analyses of complex mixtures and characterization of ions increasingly involve gas-phase separations by ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) based on the difference of ion mobility in strong and weak electric fields. The key advantage of FAIMS is substantial orthngonality to mass spectrometry (MS), which makes FAIMS/MS hybrid a powerful analytical platform of broad utility. However, the potential of FAIMS has been constrained by limited resolution. Here, we report that the use of gas mixtures comprising up to 75% He dramatically increases the FAIMS separation capability, with the resolving power for peptides and peak capacity for protein digests reaching and exceeding 100. The resolution gains extend to small molecules, where previously unresolved isomers can now be separated. These performance levels open major new applications of FAIMS in proteomic and other biomolecular analyses. 10.1021/ac902852a

Details

Language :
English
ISSN :
00032700
Volume :
82
Issue :
6
Database :
Gale General OneFile
Journal :
Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.236248057