Highly discriminatory single-nucleotide polymorphism interrogation of Escherichia coli by use of allele-specific real-time PCR and eBURST analysis

A single-nucleotide polymorphism (SNP) genotyping approach was applied to differentiate Escherichia coli isolates from six different animal species in Southeast Queensland (SEQ), Australia. The results confirmed the potential application of SNP typing as a promising method for the discrimination of host-specific groups and allows for the identification of human-specific E. coli in environmental samples.