Display of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier

The studies have shown that the inner-coat oxalate decarboxylate (OxdD) has exposed an endogenous phytase, a commonly used feed for monogastric animals, in an active form at the spore surface. The potential of OxdD as a carrier protein is documented through the spore display of a bioactive heterologous passenger, the tetrameric [beta]-glucuronidase enzyme from Escherichia coli.