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ATM controls meiotic double-strand-break formation

Authors :
Lange, Julian
Pan, Jing
Cole, Francesca
Thelen, Michael P.
Jasin, Maria
Keeney, Scott
Source :
Nature. November 10, 2011, Vol. 479 Issue 7372, p237, 5 p.
Publication Year :
2011

Abstract

In many organisms, developmentally programmed double-strand breaks (DSBs) formed by the SPO11 transesterase initiate meiotic recombination, which promotes pairing and segregation of homologous chromosomes (1). Because every chromosome must receive a minimum number of DSBs, attention has focused on factors that support DSB formation (2). However, improperly repaired DSBs can cause meiotic arrest or mutation (3,4); thus, having too many DSBs is probably as deleterious as having too few. Only a small fraction of SPO11 protein ever makes a DSB in yeast or mouse (5) and SPO11 and its accessory factors remain abundant long after most DSB formation ceases (1), implying the existence of mechanisms that restrain SPO11 activity to limit DSB numbers. Here we report that the number of meiotic DSBs in mouse is controlled by ATM, a kinase activated by DNA damage to trigger checkpoint signalling and promote DSB repair. Levels of SPO11-oligonucleotide complexes, by-products of meiotic DSB formation, are elevated at least tenfold in spermatocytes lacking ATM. Moreover, Atm mutation renders SPO11-oligonucleotide levels sensitive to genetic manipulations that modulate SPO11 protein levels. We propose that ATM restrains SPO11 via a negative feedback loop in which kinase activation by DSBs suppresses further DSB formation. Our findings explain previously puzzling phenotypes of Atm-null mice and provide a molecular basis for the gonadal dysgenesis observed in ataxia telangiectasia, the human syndrome caused by ATM deficiency.<br />SPO11 creates DSBs via a covalent protein-DNA intermediate that is endonucleolytically cleaved to release SPO11 attached to a short oligonucleotide, freeing DSB ends for further processing and recombination (5) (Fig. [...]

Details

Language :
English
ISSN :
00280836
Volume :
479
Issue :
7372
Database :
Gale General OneFile
Journal :
Nature
Publication Type :
Academic Journal
Accession number :
edsgcl.273195065
Full Text :
https://doi.org/10.1038/nature10508