Roles for C-X-C chemokines and C5a in lung injury after hindlimb ischemia-reperfusion

A study was conducted to analyze the functions of the C-X-C chemokines cytokine-induced neutrophil chemoattractant and macrophage inflammatory protein-2 in the development of lung injury after hindlimb ischemia-reperfusion in rats. Normal human blood was obtained in vials supporting citrate. Neutrophils were then determined by Ficoll and dextran sedimentation. Moreover, data were examined using a one-way analysis of variance.