Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria

A study was conducted to analyze the diversity of predominant bacteria in the human gastrointestinal tract. 16S rRNA-based approaches were utilized to carry out the analysis. Different profiles were determined using temperature gradient gel electrophoresis. Results indicated the effectiveness of the combination of cloning and temperature gradient gel electrophoresis analysis in evaluating rDNA amplicons and observing microbial communities in feces.