Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples

A study reported the application of new primer systems for the nirK and nirS nitrite reductase genes through a system developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with polymerase chain reaction. Results suggested the suitability of the method for the qualitative discovery of denitrifying bacteria in environmental samples. This was exhibited by applying one generally amplifying primer combination for each nir gene developed.