Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberelltin deactivation

A major catabolic pathway for the gibberellins (GAs) is initiated by 2[Beta]-hydroxylation, a reaction catalyzed by 2-oxoglutarate-dependent dioxygenases. To isolate a GA 2[Beta]-hydroxylase cDNA clone we used functional screening of a cDNA library from developing cotyledons of runner bean (Phaseolus coccineus L.) with a highly sensitive tritium-release assay for enzyme activity. The encoded protein, obtained by heterologous expression in Escherichia coli, converted [GA.sub.9] to [GA.sub.51] (2[Beta]-hydroxy[GA.sub.9]) and [GA.sub.51]-catabolite, the latter produced from [GA.sub.51] by further oxidation at C-2. The enzyme thus is multifunctional and is best described as a GA 2-oxidase. The recombinant enzyme also 2[Beta]-hydroxylated other [C.sub.19]-GAs, although only [GA.sub.9] and [GA.sub.4] were converted to the corresponding catabolites. Three related cDNAs, corresponding to gene sequences present in Arabidopsis thaliana databases, also encoded functional GA 2-oxidases. Transcripts for two of the Arabidopsis genes were abundant in upper stems, flowers, and siliques, but the third transcript was not detected by Northern analysis. Transcript abundance for the two most highly expressed genes was lower in apices of the GA-deficient ga1-2 mutant of Arabidopsis than in wild-type plants and increased after treatment of the mutant with [GA.sub.3]. This up-regulation of GA 2-oxidase gene expression by GA contrasts GA-induced down-regulation of genes encoding the biosynthetic enzymes GA 20-oxidase and GA 3[Beta]-hydroxylase. These mechanisms would serve to maintain the concentrations of biologically active GAs in plant tissues.