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Mutational analysis of the phoD promoter in Bacillus subtilis: implications for PhoP binding and promoter activation of Pho regulon promoters
- Source :
- Journal of Bacteriology. April, 1999, Vol. 181 Issue 7-8, p2017, 9 p.
- Publication Year :
- 1999
-
Abstract
- Oligo-directed mutagenesis, DNase I footprinting and in vivo transcription assays have been used in studying the regulatory region of the phoD promoter in Bacillus subtilis. Results indicate that a dimer of Pho-P can stably bind two consensus repeats while PhoP binding is highly cooperative within the core promoter region. It has also been found out that specific bases made up of TT(A/T/C)ACA consensus are necessary for transcriptional activation. The spacing between two consensus repeats within a putative dimer binding site in the core region is important for PhoP binding and promoter activation.
Details
- ISSN :
- 00219193
- Volume :
- 181
- Issue :
- 7-8
- Database :
- Gale General OneFile
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.54577559