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Use of a Microplate Scintillation Counter as a Radioactivity Detector for Miniaturized Separation Techniques in Drug Metabolism
- Source :
- Analytical Chemistry. August 15, 2000, Vol. 72 Issue 16, p3956, 4 p.
- Publication Year :
- 2000
-
Abstract
- In miniaturized separation techniques, such as capillary electrophoresis (CE) or capillary liquid chromatography (LC), conventional on-line radioactivity detection of labeled compounds is restricted, because of insufficient sensitivity. It will be shown that a microplate scintillation counter for 96-well plates (TopCount) can be used as a sensitive and easy-to-handle radioactivity detector for capillary LC and CE. The attractive combination of capillary LC, eluent fractionation, and subsequent off-line counting is described. The new method is applied for rapid and sensitive separation and detection of (super 3)H-labeled parent drug and its metabolites at levels between 25 and 700 cpm in rat urine. The advantages of capillary LC coupled to the TopCount, and combined with LC-MS data, can be of benefit in many analytical areas, including the characterization of metabolites at low concentration within complex biological fluids. With the same setup, the fractionation with subsequent off-line counting is equally applicable to CE. This is demonstrated with electrophoretically separated (super 14)C-labeled impurities, nicely resolved from a negatively charged main compound, at low levels.
Details
- ISSN :
- 00032700
- Volume :
- 72
- Issue :
- 16
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.67270298