Solid-Phase Extraction Assisted Dispersive Liquid-Liquid Microextraction of Pyrene and Phenanthrene

Zinc oxide nanoparticles-chitosan assisted dispersive liquid-liquid microextraction (DLLME) was utilized to microextraction of ultra-traces of pyrene and phenanthrene from seawater samples of Chabahar Bay prior to HPLC analysis with UV detection. The analytes dispersed into organic phase with the assistance of zinc oxide nanoparticles-chitosan in the modified DLLME method. The effect of factors on extraction efficiency, including amount of zinc oxide nanoparticles-chitosan, amount of chitosan, amount of zinc oxide nanoparticles, type and volume of extraction and dispersive solvents, extraction time and rate, and time of centrifugation, was optimized and investigated. Under optimized conditions, good linearity ranges of 1.0-150 [micro]g/L for phenanthrene and 5-75 [micro]g/L for pyrene were achieved. Based on standard deviation to slope of calibration curve ratio of 3, the limits of detection were 0.4 and 1.7 [micro]g/L for phenanthrene and pyrene, respectively. Relative standard deviation was between 1.8-8.8%. The protocol was successfully utilized to the determination of pyrene and phenanthrene in seawater samples of Chabahar Bay. Relative recoveries from 79.0 to 105.0% were observed for the spiked seawater samples.
Author(s): Morteza Ziyaadini [sup.1] , Sayyed Hossein Hashemi [sup.1] , Mir Mahdi Zahedi [sup.1] , Balall Raisi [sup.1] , Soheila Bashande [sup.1] Author Affiliations: (1) grid.459445.d, 0000 0004 0481 4546, [...]