Specific Features of the Determination of Xanthophyll Esters under Reversed-Phase HPLC Conditions

Specific features of chromatographic determination of xanthophyll esters are studied using an example of marigold flower lutein diesters under reversed-phase HPLC conditions. The developed two-column method made it possible to establish that, in samples with a low solubility of carotenoids in the used solvent and on a chromatography column in using a mobile phase with a low solubility of carotenoids, the precipitation of diesters is possible, which detrimentally affects the accuracy of the chromatographic determination. A critical factor in this case isì temperature: the storage of samples (solutions) in a refrigerator is not always advisable, because freezing of the main components is possible. It was shown that the use of a mobile phase containing from 0 to 10 vol % acetonitrile in acetone at a temperature not lower than 20°C is acceptable for the separation of all-trans-lutein diesters from cis-derivatives of lutein and zeaxanthin derivatives on 'monomeric' C18-phases.
Author(s): V. I. Deineka [sup.1] , T. G. Burzhinskaya [sup.1] , I. P. Blinova [sup.1] , L. A. Deineka [sup.1] Author Affiliations: (1) grid.445984.0, 0000 0001 2224 0652, Institute of [...]