Properties of the major non-specific endonuclease from the strict anaerobe Fibrobacter succinogenes and evidence for disulfide bond formation in vivo

The major non-specific endonuclease from the strict anaerobe Fibrobacter succinogenes has been studied with the finding of evidence for disulfide bond formation in vivo. Magnesium was found to be required for nucleolytic activity, although calcium was not. Manganese ions substituted for magnesium, giving, however, less stimulation. The first indication has been found in a strict anaerobe for a functional periplasmic disulfide bond forming system, similar phenotypically to Dsb systems in facultative and aerobic bacteria.